The discovery of DsRed (and other Anthozoa fluorescent proteins of various hues) had a twofold impact on the fluorescent protein engineering community. First, there is now a relatively diverse ...

and yellow fluorescent protein (YFP) 2. Figure 1: ATP concentration affects signal efficacy of CFP-YFP–based FRET sensors. It is important to note further that the ratio changes (8–45% change ...

This approach is called homoFRET-FP and can be used to analyze and quantify the formation of homodimers and oligomers of the same protein. Till now, homo-FRET has been rarely used in high-throughput ...